Cyclosporine a drug-delivery system for high-risk penetrating keratoplasty: Stabilizing the intraocular immune microenvironment.

摘要: Cyclosporine A (CsA) is an essential medication used to prevent corneal allograft rejection. Our preliminary studies revealed that CsA drug-delivery system (DDS) was more effective in preventing high-risk rejection than topical application. However, the impacts of DDS on intraocular immune microenvironment were not fully elucidated. In present study, we investigated effect cornea allograft, aqueous humor, and iris-ciliary body using a rabbit model penetrating keratoplasty. New Zealand white rabbits divided into four groups: normal control group, untreated eye drop group group. Graft survival monitored for 12 weeks, therapeutic effects evaluated at 3 weeks after mean graft time significantly prolonged when compared with groups. At all time-points, Langerhans cell density, inflammatory central thickness much lower(all p < 0.01) groups, which their parameters higher (all 0.01). Compared implanted markedly decreased CD11b+ CD8+ T infiltration grafts. treatment also greatly reduced CD4+ density expression interferon-gamma, interleukin-2 (IL-2), IL-6, CD80, CD86 mRNA both Moreover, IL-2 level humor (p Taken together, our results suggest anterior chamber create relative immunosuppressive graft, body, humor. Stabilizing could partially elucidate mechanism suppressing