Analysis of Th1 and Th2 cells in murine gut-associated tissues. Frequencies of CD4+ and CD8+ T cells that secrete IFN-gamma and IL-5.

摘要: After Ag and/or mitogen stimulation, cloned mouse Th1 and Th2 cells produce different cytokines that contribute to induction of particular B cell isotype responses. In this regard, IL-5 produced by has been shown enhance IgA synthesis in LPS-triggered splenic (SP) or unstimulated Peyer's patch (PP) cultures. This raises the possibility may occur higher frequency gut-associated tissues, because these areas are committed synthesis. We have used an ELISPOT assay detect individual T producing IFN-gamma IL-5. For assay, mAb TRFK-5 biotinylated TRFK-4 were coating detection, respectively, whereas R4-6A2 XMG 1.2 similarly for enumeration IFN-gamma-specific spot forming (SFC). Specificity each was tested using Con A-activated, (H66-61) (CDC-25) cells, where only SFC gave IL-5-specific spots. Further, preincubation with rIL-5 IFN-gamma, abrogated formation specific spots when A-activated SP CD4+ cells. Both de novo, treatment cycloheximide inhibited both SFC. assessed numbers spontaneously secreting PP lamina propria intraepithelial lymphocyte (LPL IEL) populations. Moderate levels occurred IEL subset, existed LPL population. Although significant (Th1-type) also seen LPLs, always (Th1:Th2 = 1:3). IELs, equal seen. Interestingly, CD8+ two cytokines. contrast, freshly isolated from PP, inductive site, contained smaller IL-5- IFN-gamma-secreting had essentially no When stimulated A, approximately IFN-gamma- IL-5-producing appeared.(ABSTRACT TRUNCATED AT 250 WORDS)