作者: Wang Shikai , Wei Xiaoyan , Zhao Jianyang
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摘要: The invention discloses a culture method for engineered Escherichia coli capable of efficiently expressing supercoiled plasmid DNA. comprises the following steps: (1) preparing fluid medium; (2) solid (3) sterilizing (4) performing pure culture; and (5) enlarged culture. According to colicapable DNA provided by invention, raw materials in medium are reasonably matched, is scientific. By virtue theculture disclosed total content bacterium liquid unit volume, cell improved, has an excellent effect promoting extraction application market popularization value.