Imaging filopodia dynamics in the mouse blastocyst.

摘要: During mammalian development, the first cell lineage diversification event occurs in blastocyst, when trophectoderm (TE) and inner mass (ICM) become established. Part of TE (polar) remains contact with ICM differs from mural (mTE) which is separated by a cavity known as blastocoele. The presence filopodia connecting cells distant through blastocoelic fluid was investigated this work. We describe two types actin-based projections found freshly dissected vitro cultured expanding blastocysts: abundant short projecting into that present continuous undulating behavior; long, thin traversing ICM. Videomicroscopy analyses revealed vesicle-like structures moving along dynamic cytoskeletal rearrangements. These observations, together immunolocalization FGFR2 ErbB3 receptors to these extensions, suggest they display signal transduction activity. propose are employed mitotic mTE receive required signals for division after