Characterization of an inhibitor of neuronal plasminogen activator released by heart cells.

摘要: A basic understanding of growth cone dynamics and developmental events involving cones requires an the function regulation molecules associated with released by cones. Rat sympathetic neurons in culture release a urokinase-like plasminogen activator from their distal processes and/or (Pittman, 1985a). When are grown cocultures heart cells, however, activity is not detected. The absence cells appears to be due inhibitor cells. This has molecular weight approximately 50 kDa presence SDS apparent weights greater than 2000 under native conditions. significant fraction large- molecular-weight form converted smaller following treatment heparinase. Extremely stable complexes 68 80 formed between activator, urokinase, such that withstand boiling SDS/mercaptoethanol. data consistent formation urokinase-inhibitor complex heparan sulfate proteoglycan proteoglycan. highly purified preparation produces 2- 3-fold increase neurite outgrowth neurons. These indicate can regulated normal target tissue this may result increased