Differential Cellular and Subcellular Expression of the Human Multifunctional Apurinic/Apyrimidinic Endonuclease (APE/ref-1) DNA Repair Enzyme

作者: Mark R. Kelley , John N. Eble , John R. Duguid , Teresa M. Wilson

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摘要: The multifunctional mammalian apurinic/apyrimidinic endonuclease (APE) is responsible for the repair of sites in DNA. In addition, this enzyme has been shown to function as a redox factor facilitating DNA-binding capability JUN and FOS, well numerous other transcription factors through alteration state. Biochemical studies organ homogenates have that APE present different tissues studied at similar concentrations. study examines immunohistochemical distribution several organs demonstrates new unexpected patterns cellular subcellular localization enzyme. hippocampus, protein was highly expressed neurons dentate gyrus regions CA3 CA4, unexpectedly, staining primarily cytoplasmic. AP immunoreactivity cerebellum found granule Purkinje cells, both cytoplasmic nuclear. hypoglossal nucleus brainstem, where motor control tongue movement reside, showed reactivity Nissl substance. Skin, liver, duodenum demonstrated nuclear staining; however, duodenum, only enterocyte nuclei proximal villus crypts Lieberkuhn were stained, with no distal villus. These results suggest regulatory functional roles cells body. This shows importance correlating vitro findings tissue culture organism whole. seen parts brain liver suggests there may be additional functions yet described.

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