A method for promoting fatty acid synthesis in a plant
作者： Yuka Sun Beam Fujinari Madoka , Yukiko A Mitsuke-momiji-en Sasaki , Akiho Yokota
DOI:
关键词:
摘要: According to the present invention, a novel method for promoting fatty acid content in plant is provided. In this method, promoter of accD gene acetyl-CoA carboxylase was replaced with directing abundant expression plastids and chloroplasts by using plastid transformation. This increases carboxyltransferase beta subunit protein encoded gene. Accompanied it, other subunits constituting apparently enzyme increases. Since key rate-limiting step synthesis, synthesis can be promoted invention. The transformed produced according invention exhibits remarkable enhancement leaves seeds. Moreover, leaf longevity extended number seeds per body increased, thereby seed oil productivity are improved.
lens.org 参考文章(10)
Joong-Kook Choi, Eve S. Wurtele, Abbaraju Hari Kishan Rao, Basil J. Nikolau, A modified arabidopsis thaliana cac1, cac2 or cac3 promoter and an arabidopsis thaliana cac1, cac2 or cac3 suppressor element and methods of use thereof ,(1999)
Y Sasaki, K Hakamada, Y Suama, Y Nagano, I Furusawa, R Matsuno, Chloroplast-encoded protein as a subunit of acetyl-CoA carboxylase in pea plant. Journal of Biological Chemistry. ,vol. 268, pp. 25118- 25123 ,(1993) , 10.1016/S0021-9258(19)74577-3
Peter T. Hajdukiewicz, Lori A. Allison, Pal Maliga, Nuclear-encoded transcription system in plastids of higher plants ,(1996)
Pal Maliga, Zora S. Maliga, Method for stably transforming plastids of multicellular plants ,(1993)
Vic C. Knauf, Elaine A. Best, Methods and compositions for modulating lipid content of plant tissues ,(1994)
Y. Sasaki, T. Konishi, Y. Nagano, The Compartmentation of Acetyl-Coenzyme A Carboxylase in Plants. Plant Physiology. ,vol. 108, pp. 445- 449 ,(1995) , 10.1104/PP.108.2.445
Hideo Nakashita, Keiko Yoshioka, Yoshiharu c, o Riken Doi, o Riken Yamaguchi, Isamu c, Methods for transformation of plants, transformed plants and processes for preparation of polyesters ,(2000)
Antoni Ryszard Slabas, Kieran Michael Elborough, Plant gene encoding acetyl coenzyme a carboxylase biotin carboxylase carrier protein ,(1996)
Y Sasaki, Enhancement of the plastidic acetyl-CoA carboxylase level using the tobacco plastid transformation Science Access. ,vol. 3, ,(2001) , 10.1071/SA0403718
John B. Ohlrogge, Basil S. Shorrosh, STRUCTURE AND EXPRESSION OF THE ALPHA-CARBOXYLTRANSFERASE SUBUNIT OF HETEROMERIC-ACETYL-CoA CARBOXYLASE ,(1997)