A Measurable Increase in Oxidative Damage Due to Reduction in Superoxide Detoxification Fails to Shorten the Life Span of Long-Lived Mitochondrial Mutants of Caenorhabditis elegans

摘要: SOD-1 and SOD-2 detoxify superoxide in the cytoplasm mitochondria. We find that, although several long-lived mutants of Caenorhabditis elegans have increased SOD levels, this phenomenon does not correlate with life span or growth rate. Furthermore, disruption sod-1 -2 expression produces numerous phenotypes, including sensitivity to paraquat oxidative damage proteins (except daf-2 mutants), fails shorten these mutants. In fact, sod-1(RNAi) increases sod-2(RNAi) that clk-1 Our results suggest detoxification low are crucial for longevity examined, possible exception daf-2, where our inconclusive. These surprising because we examined specifically affect mitochondrial electron transport, a process whose involvement life-span determination is believed be related generation. discuss significance findings light stress theory aging.