PCR and DNA Hybridization Methods for Specific Detection of Bean-Infecting Begomoviruses in the Americas and Caribbean

摘要: Begomoviruses are a major problem for common bean production in the tropics and subtropics of Americas Caribbean. Multiplex polymerase chain reaction (PCR) primer pairs nucleic acid hybridization probes have been developed to differentiate five bean-infecting begomoviruses were used assay reference field-collected samples from Florida, Mexico, Central America, Caribbean, Brazil. Bean golden mosaic virus was found Brazil, calico yellow Florida. dwarf not detected any field samples. Tomato leaf curl only tomato These detection methods will provide tools assist understanding epidemiology diversity geminiviruses as well facilitate resistance breeding, cultivar selection, development strategies control.