Interleukin 1-dependent induction of both interleukin 2 secretion and interleukin 2 receptor expression by thymoma cells.

摘要: The macrophage-derived product, interleukin 1 (IL 1) is thought to play an important regulatory role in the proliferation of T lymphocytes; however, its mechanism action unknown. We describe this report a variant subline EL4 thymoma cells (EL4-6.1) that displays high degree responsiveness IL 1. show recombinant can induce both secretion 2 2) and expression receptors 2-R) by these cells. EL4-6.1 do not constitutively secrete 2, nor they express 2-R; but when cultured presence 1, detectable amounts (5 15 U/ml). In either suboptimal levels phorbol ester (PMA) or Ionomycin, addition resulted up 80-fold enhancement amount secreted. Stimulation with alone combination Ionomycin was unable 2-R However, concentrations PMA, induced about 3000 affinity (dissociation constant, Kd 31 pM) 50,000 low (Kd 2800 2-R. These were functional, based on their ability rapidly internalize 2. This model system will allow detailed analysis mechanisms involved regulation immune response