Detection and Identification of Bacteriovorax stolpii UKi2 Sphingophosphonolipid Molecular Species

摘要: Bacteriovorax stolpii is a predator of larger gram-negative bacteria and lives as parasite in the intraperiplasmic space host cell. This bacterium unusual among prokaryotes that sphingolipids comprise large proportion its lipids. We here report presence 18 molecular species B. UKi2 sphingophosphonolipids (SPNLs). 31P NMR spectroscopy analysis Pi released by differential hydrolysis protocol confirmed phosphonyl nature these The SPNLs were dominated those with 1-hydroxy-2-aminoethane phosphonate (hydroxy-aminoethylphosphonate) polar head groups; aminoethylphosphonate was also detected minor SPNL components. long-chain bases (LCBs) C17 iso-branched phytosphingosine; dihydrosphingosine present some SPNLs. N-linked fatty acids predominantly most contained an α-hydroxy group (C15α-hydroxy acid major acid). Minor containing nonhydroxy detected. definitive iso-structures predominant LCBs established using 13C 3H nuclear magnetic resonance spectroscopy; less than 20% unbranched. Detection analyses intact compounds MS-MS performed hybrid quadrupole time-of-flight (Q-TOF-II) MS equipped electrospray ionization source. Analyses peracetylated derivatives verified structural assignments