Aberrant expression and regulation of NR2F2 and CTNNB1 in uterine fibroids.

摘要: Uterine fibroids are the most common benign tumour afflicting women of reproductive age. Despite large healthcare burden caused by fibroids, there is only limited understanding molecular mechanisms that drive fibroid pathophysiology. Although a number genes differentially expressed in compared with myometrium, it likely these differences consequence presence and not causal. The aim this study was to investigate expression regulation NR2F2 CTNNB1 based on their potential causal role uterine We used real-time quantitative RT-PCR, western blotting immunohistochemistry describe matched human myometrial tissues. Primary smooth muscle cell cultures were treated progesterone and/or retinoic acid (RA) sonic hedgehog (SHH) conditioned media regulatory pathways for proteins. showed aberrantly tissue strong blood vessel-specific localisation. SHH pathway shown be active primary cultures, did regulate or mRNA expression. However, RA combined regulated mRNA, but CTNNB1, cultures. In conclusion, we demonstrate aberrant normal consistent hypothesis factors may play development.