Biomolecule localization and surface engineering within size tunable nanoporous silica particles

摘要: OF DISSERTATION BIOMOLECULE LOCALIZATION AND SURFACE ENGINEERING WITHIN SIZE TUNABLE NANOPOROUS SILICA PARTICLES Mesoporous silica materials are versatile platforms for biological catalysis, isolation of small molecules detection and separation applications. The design mesoporous supports tailored protein biomolecule interactions has been limited by the techniques to demonstrate location functionality as a function pore size. This work examines interaction proteins lipid bilayers with engineered porous surfaces using spherical particles tunable diameters (3 – 12 nm) in range relevant uptake pores, large particle sizes (5 15 μm) amenable microscopy imaging differentiation between external surface within pore, important applications requiring protection or catalytic activity is demonstrated. A protease / fluorescent system used investigate size, indicating narrow size capable protection, slightly larger than interest approaching dimensions. Selective functionalization, this case exterior-only functionalization amines, extended materials. reaction time dependent approach demonstrated first visually confirmed, selective amine method accessible supports. nanoparticles effective bilayer membranes membrane associated separations therapeutic delivery, although role support porosity on fluidity unknown. Transport properties filled diameter measured time. Bilayer diffusivity increases increasing independent core, mid cap particle, suggesting uniform long mobility pores. Application was examined unique adhere functional established vesicles derived from cell plasma their proteins. investigation retains native membranes, stabilizing