Antitumor activities of human autologous cytokine-induced killer (CIK) cells against hepatocellular carcinoma cellsin vitroandin vivo

摘要: AIM: To characterize the anticancer function of cytokine-induced killer cells (CIK) and develop an adoptive immunotherapy for patients with primary hepatocellular carcinoma (HCC), we evaluated proliferation rate, phenotype antitumor activity human CIK from healthy donors HCC in vitro vivo. METHODS: Peripheral blood mononuclear (PBMC) were incubated induced into presence various cytokines such as interferon-gamma (IFN-γ), interleukin-1 (IL-1), IL-2, monoclonal antibody (mAb) against CD3. The characterization identified by flow cytometric analysis. cytotoxicity was determined 51Cr release assay. RESULTS: shown to be a heterogeneous population different cellular phenotypes. percentage CD3+CD56+ positive cells, dominant effector total patients, significantly increased 0.1%-0.13% at day 0 19.0%-20.5% 21 incubation, which suggested that proliferated faster than other cell populations protocol used this study. After 28 more 300-fold 500-fold number, respectively. originated possessed higher cytotoxic on autologous lymphokine-activated (LAK) PBMC cells. In vivo animal experiment, had stronger effects inhibition tumor growth Balb/c nude mice bearing BEL-7402-producing LAK (mean inhibitory 84.7% vs 52.8%, P < 0.05) or 37.1%, 0.01). CONCLUSION: Autologous are highly efficient might serve alternative therapeutic strategy patients.