作者: Maryam Modarai , Andy Suter , Andreas Kortenkamp , Michael Heinrich
DOI: 10.1111/J.2042-7158.2010.01235.X
关键词:
摘要: Objectives Potential interactions between herbal medicinal products and the cytochrome (CYP) P450 system are an important safety concern. We set out to develop a screening panel for assessing such use it evaluate interaction potential of devil's claw. Methods The consisted luminescence-based inhibition assays CYP1A2, 2C9, 2C19, 2D6 3A4, reporter gene (luciferase) assay pregnane X receptor (PXR) activation CYP3A4 induction. Caftaric acid chlorogenic acid, two compounds with strong fluorescence quenching properties, were used demonstrate assay's resistance interference. tested 10 commercial claw preparations as well harpagoside harpagide, constituents claw. Key findings Five found weakly inhibit (IC50 124.2–327.6 µg/ml) five activate PXR (EC50 10.21–169.3 µg/ml). Harpagoside harpagide did not CYP3A4. In agreement published data, bergamottin, natural product known interact CYP3A4, was shown IC50 13.63 µm EC50 6.7 µm. Conclusions Devil's unlikely have clinically relevant effect on CYP function. proved effective in preparations, demonstrating its suitability plant extracts. It showed superior sensitivity