Discrete Turing patterns in coupled reaction cells in a cyclic array

摘要: Our work is focused on the experimental observation of non-uniform stationary states (discrete Turing patterns) in a cyclic coupled cell array with glycolytic oscillatory reaction. Mutual mass exchange between two stirred reaction cells realized by using peristaltic pump delivering reciprocally the mixture both directions. The are fed solutions yeast extract and d-glucose. system formed tubing, connecting cells, acting as additional reactors because non-negligible transport time. Experiments conducted under 45 50 °C to thermally activate positive negative feedback loops enzyme phosphofructokinase thus achieve conditions appropriate for occurrence patterns. dynamical state one ring measured UV–Vis spectrophotometer reading absorbance levels adenosine pool together NAD+ (260 nm) level NADH (340 nm). We report transitions uniform states, oscillations. also performed an analysis stability bifurcations model four core kinetic glycolysis. By examining space relevant parameters, we found discrete patterns consistent our experiments. Solution diagrams used display concentration ADP function inhibition rate coefficient $$\sigma_{inh}$$ explain mechanism which occur.