Population-Sequencing as a Biomarker for Sample Characterization

摘要: Sequencing is accepted as the “gold” standard for genetic analysis and continues to be used a validation reference tool. The idea of using sequence directly sample characterization has been met with skepticism. However, herein, utility direct use sequencing identify multiple genomes present in samples presented reviewed. All “pure” isolates are populations genomes. Population-Sequencing probabilistic matching tools combination large volumes information present, based on DNA across entire determine genome assignments, calculate confidence scores major minor content. Accurate identification from mixtures without culture purification steps can achieve phylogenetic classification by millions fragments. Genome data function biomarkers interrogate content establish profile, inclusive components, drill down rare SNP mutation events, compare relatedness between samples, profile-to-profile, provide or statistical scoring attribution. application will facilitate strategies.