High-Precision, Whole-Genome Sequencing of Laboratory Strains Facilitates Genetic Studies

摘要: Whole-genome sequencing is a powerful technique for obtaining the reference sequence information of multiple organisms. Its use can be dramatically expanded to rapidly identify genomic variations, which linked with phenotypes obtain biological insights. We explored these potential applications using emerging next-generation platform Solexa Genome Analyzer, and well-characterized model bacterium Bacillus subtilis. Combining experimental verification, we first improved accuracy published B. subtilis strain 168, then obtained sequences related laboratory strains different isolates each strain. This provides framework comparing divergence between their individual isolates. also demonstrated power by its results predict defect in citrate signal transduction pathway common strain, verified experimentally. Finally, examined molecular nature spontaneously generated mutations that suppress growth caused deletion stringent response mediator relA. Using whole-genome sequencing, mapped suppressor two small homologs Interestingly, stable had both genes, mutation alone partially relieving relA defect. supports an intriguing three-locus interaction module not easily identifiable through traditional mapping. conclude drastically accelerate identification complex genetic interactions, it applied as standard tool investigate traits