Transcriptome analysis reveals seven key immune pathways of Japanese flounder (Paralichthys olivaceus) involved in megalocytivirus infection.

摘要: Megalocytivirus is a serious viral pathogen to many farmed fish including Japanese flounder (Paralichthys olivaceus). In this study, in order systematically identify host immune genes induced by megalocytivirus infection, we examined the transcription profiles of infected for 2, 6, and 8 days. Compared with uninfected fish, virus-infected exhibited 1242 differentially expressed (DEGs), 225, 275, 877 DEGs occurring at days post respectively. Of these DEGs, 728 were upregulated 659 downregulated. The majority time-specific formed four distinct expression well correlated time infection. classified into diverse Gene Ontology (GO) functional terms enriched 27 Kyoto Encyclopedia Genes Genomes (KEGG) pathways, approximately one third which related immunity. Weighted co-expression network analysis (WGCNA) was used 16 key belonging seven pathways (RIG-I-like receptor signaling pathway, JAK-STAT TLR cytokine-cytokine interaction, phagosome, apoptosis, p53 pathway). These interacted extensively complicated networks. This study provided global picture megalocytivirus-induced gene transcriptome level uncovered set closely linked results targets future delineation factors implicated anti-megalocytivirus immunity flounder.