Subtype-specific regulation of muscarinic receptor expression and function by heterologous receptor activation.
作者： Darrell A. Jackson , Neil M. Nathanson
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摘要: Incubation of cultured embryonic chicken heart cells with the beta-adrenergic agonist isoproterenol resulted in a dose-dependent increase number mAChR on surface intact cells. The isoproterenol-mediated was time dependent and reached maximum by 48 h. Chick treated exhibited greater than 6-fold sensitivity for carbachol-mediated inhibition adenylyl cyclase activity as compared to control. Stimulation 24 h 25-35% cm2 mRNA levels control levels. In contrast, level cm4 not significantly affected treatment. were maximally elevated 15 following stimulation remained up 72 presence Rp-cAMP, an inhibitor cAMP-dependent protein kinase, blocked mRNA. Thus, prolonged activation receptors results muscarinic responsiveness chick due kinase mediated
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