IL-1 beta but not the NALP3 inflammasome is an important determinant of endothelial cell responses to necrotic/dangerous trophoblastic debris

摘要: Abstract Introduction Necrotic but not apoptotic trophoblastic debris can induce endothelial cell activation the mechanism by which cells distinguish from necrotic is unclear. The NALP3 inflammasome a pattern recognition receptor that macrophages employ to recognise “danger signals” in corpses. In this study, we hypothesized identify and respond via inflammasome. Methods effect of on expression components was investigated using qRT-PCR, immunoassays fluorescent caspase 1 activity assay. IL-1β quantified ELISA. Endothelial measured surface ICAM monocytes adhesion Results expressed resting vascular involved response danger signals. However, exposure did significantly alter any three at mRNA level, nor caspase-1 increased. Conditioned media exposed contained elevated levels derived contributed activation. Discussion induced through IL-1β/IL-1R pathway. process.