Comparative Genomic Hybridization in the Detection of DNA Copy Number Abnormalities in Uveal Melanoma

摘要: Genomic instability appears to play an important role in the development, growth, invasiveness, and eventual metastasis of neoplastic cell. We have used a powerful new technique, comparative genomic hybridization, evaluate genetic alterations 10 fresh frozen uveal melanomas. Comparative hybridization utilizes dual fluorescence situ characterize chromosome deletions duplications, allowing for simultaneous evaluation entire human genome. Several consistent chromosomal abnormalities were detected. This study confirmed previous findings obtained using standard cytogenetic techniques but demonstrated increased incidence chromosomes 3 8; there was loss duplication 8q. In addition, we identified, although less frequently, other recurrent abnormal regions including on 6p, 7q, 9p, 13q.