Development of the sequence-characterized amplified region (SCAR) marker for distinction of intergeneric hybrids between Argyranthemum frutescens (L.) Sch. Bip. and Rhodanthemum gayanum (Cross. & Durieu) B.H. Wilcox, K. Bremer & Humphries.

作者: Takahiro Muto , Takanori Kuronuma , Masaya Ando , Hiroyuki Katsuoka , Zentaro Inaba

DOI: 10.5511/PLANTBIOTECHNOLOGY.19.1104A

关键词:

摘要: Distinction of intergeneric hybrids between Argyranthemum frutescens (L.) Sch. Bip. and Rhodanthemum gayanum (Cross. & Durieu) B.H. Wilcox, K. Bremer Humphries is important for the development new hybrids. The aim this study was to develop a simple cost-effective DNA marker distinguish large number putative A. × R. during primary selection. We designed sequence-characterized amplified region (SCAR) markers based on genetic sequences internal transcribed spacer (ITS) gayanum. Specific SCAR bands in but not In contrast, specific frutescens. Additionally, we confirmed reproducibility using 31 cultivars. findings demonstrated that these could be used This first report facilitate distinction

参考文章(24)
Loredana Mariniello, Maria Grazia Sommella, Angela Sorrentino, Marcello Forlani, Raffaele Porta, Identification of Prunus armeniaca cultivars by RAPD and SCAR markers Biotechnology Letters. ,vol. 24, pp. 749- 755 ,(2002) , 10.1023/A:1015516712754
Tao Cheng, Chao Xu, Li Lei, Changhao Li, Yu Zhang, Shiliang Zhou, Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity Molecular Ecology Resources. ,vol. 16, pp. 138- 149 ,(2016) , 10.1111/1755-0998.12438
I. Paran, R. W. Michelmore, Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce Theoretical and Applied Genetics. ,vol. 85, pp. 985- 993 ,(1993) , 10.1007/BF00215038
Tsuyoshi Motohashi, Miyoko I. Toda, Katsuhiko Kondo, Adventitious embryo formation derived from zygotic embryos in Cycas revoluta Plant Biotechnology. ,vol. 25, pp. 589- 591 ,(2008) , 10.5511/PLANTBIOTECHNOLOGY.25.589
IJFW Álvarez, JF14615184 Wendel, None, Ribosomal ITS sequences and plant phylogenetic inference. Molecular Phylogenetics and Evolution. ,vol. 29, pp. 417- 434 ,(2003) , 10.1016/S1055-7903(03)00208-2
Elizabeth A. Scheef, Michael D. Casler, Geunhwa Jung, Development of Species-Specific SCAR Markers in Bentgrass Crop Science. ,vol. 43, pp. 345- 349 ,(2003) , 10.2135/CROPSCI2003.3450