Application of original assemblies of polyelectrolytes, urease and electrodeposited polyaniline as sensitive films of potentiometric urea biosensors

作者: Cédric C. Buron , Mélanie Quinart , Tjasa Vrlinic , Sami Yunus , Karine Glinel

DOI: 10.1016/J.ELECTACTA.2014.10.026

关键词:

摘要: Original assemblies were prepared for use as sensitive films of potentiometric enzyme urea sensors, and compared to identify the more efficient structure with respect stability. These included electrodeposited polyaniline, used transducer, urease, catalyst, biocompatible polyelectrolytes, a matrix preserve integrity in film. Two kinds done: first one consisted adsorption urease onto polyaniline film followed by chitosan-carboxymethylpullulan multilayer film, while second urease-chitosan an The morphological features growth these characterized scanning electron microscopy quartz crystal microbalance, respectively. This allowed us demonstrate that are successfully formed electrodes sensors. responses both then measured function concentration using home-made portable potentiostat. electrochemical response resulting sensors was fast types assemblies, but stability time much better obtained from alternative chitosan layer adsorbed over polyaniline.

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