Accuracy of three automated 25-hydroxyvitamin D assays in hemodialysis patients.

摘要: Abstract Introduction We evaluated the accuracy of three automated assays for 25(OH)D measurement in comparison to ID-XLC-MS/MS hemodialysis patients, considering importance their vitamin D status and reported discrepant results obtained with assays. Methods All were heterogeneous, competitive immunoassays or binding protein on Architect (Abbott), Modular E170 (Roche) iSYS (IDS). Measurements performed serum 99 patients 50 healthy subjects, double blind a different operator aliquot each method. Results showed highest deviation (slope 0.3864, intercept 8.7409) subjects 0.5024, 6.8426) significant lower results. Considering 30 ng/ml as cut-off optimal concentration, falsely assigned 48.5% 6% subgroup suboptimal status. also deviated 0.6136, 8.6604) but less discordant values than concentrations between 10 40 ng/ml. Conclusion conclude that not all may be considered equally accurate samples from compared subjects. found most deviating Abbott (Architect) measurements well suggest possible role matrix effects like elevated urea other retained metabolites sera, causing incomplete disruption DBP, poor assay accuracy.