Epidermal Growth Factor
作者： John M. Taylor , William M. Mitchell , Stanley Cohen
DOI: 10.1016/S0021-9258(19)44847-3
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摘要: Abstract The major physicochemical properties of epidermal growth factor are reported. molecular weight is estimated to be approximately 6100 by the methods sedimentation equilibrium, gel filtration, and minimum calculations from amino acid analyses. Epidermal a single chain polypeptide having an asparagine amino-terminal residue arginine carboxyl-terminal residue. It further characterized absence 3 specific residues: lysine, alanine, phenylalanine. No detectable free sulfhydryl groups, hexosamines, or neutral sugars were observed. has isoelectric point pH 4.60. ultraviolet absorption spectrum typical for proteins, with E1%1 cm at 280 nm 30.9. secondary structure, as judged circular dichroism, principally nonhelical.
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