Endocytosis and dissociation of class I MHC molecules labeled with fluorescent beta-2 microglobulin.

摘要: Membrane class I MHC molecules of Con-A activated and lymphoma murine cells have been labeled by exchange the cell's beta 2m with soluble fl-beta 2m. It has previously shown that this method labeling is specific does not affect biologic properties Ag. With it possible to demonstrate constitutive endocytosis fluorescence microscopy measuring resistance quenching crystal violet internalized molecules. We could also follow kinetics dissociation from at different pH. At pH 5.5, average endosomes, there considerable within 15 20 min, recycling half time containing endosomes in T cells. Inasmuch as process reversible likely that, cells, undergo conformational changes going off on consequent peptide binding site. This might be involved Ag presentation, but, because apparently limited would play a role presentation own TCR idiotypic interactions between