Vasculotropic, paracrine actions of infused mesenchymal stem cells are important to the recovery from acute kidney injury.

摘要: Acute kidney injury (AKI) is a major clinical problem in which critical vascular, pathophysiological component recognized. We demonstrated previously that mesenchymal stem cells (MSC), unlike fibroblasts, are significantly renoprotective after ischemia-reperfusion and concluded this renoprotection mediated primarily by paracrine mechanisms. In study, we investigated whether MSC possess vasculoprotective activity may contribute, at least part, to an improved outcome AKI. MSC-conditioned medium contains VEGF, HGF, IGF-1 augments aortic endothelial cell (EC) growth survival, response not observed with fibroblast-conditioned medium. EC share vasculotropic gene expression profiles, as both form capillary tubes vitro on Matrigel alone or cooperation without fusion. undergo differentiation into endothelial-like phenotype culture develop vascular structures vivo. Infused were readily detected the early reflow but only rarely engrafted 1 wk post-AKI. attached renal microvascular circulation decreased apoptosis of adjacent cells. Infusion immediately severe AKI did improve blood flow, renovascular resistance, outer cortical flow. These data demonstrate unique vasculotropic, actions elicited play significant role AKI, further demonstrating therapy has promise novel intervention