Label‐free mass spectrometry‐based quantification of hemagglutinin and neuraminidase in influenza virus preparations and vaccines

摘要: Background Influenza vaccination is the primary method for preventing influenza and its severe complications. An accurate rapid to determine hemagglutinin (HA) concentration would facilitate reference antigen preparation consequently expedite availability of seasonal as well pandemic vaccines. Objective The goal this study was develop a label-free mass spectrometry (MS) based that enables simultaneous identification quantification HA, neuraminidase (NA), other viral proteins protein contaminations in vaccine or virus preparations. Methods presented on LC ⁄MSE analysis preparations tryptic digests spiked with known amount standard from which universal response factor generated applied calculate identified mixture. Results We show that, use an appropriate internal standard, MS-based applicable absolute HA relative impurities vaccines different subtype recombinant preferred provides most results HAs proteins. measure quantity quantities whole monovalent vaccine, providing data demonstrate strain-dependent differences NA.