Oxidative species and S-glutathionyl conjugates in the apoptosis induction by allyl thiosulfate

摘要: Natural allyl sulfur compounds show antiproliferative effects on tumor cells, but the biochemical mechanisms underlying antitumorigenic properties of organ are not yet fully understood. Sodium 2-propenyl-thiosulfate is a garlic water-soluble organo-sulfane compound able to promote apoptosis in cancer cells, affecting ‘managing’ of redox state cell. Our studies that sodium 2-propenylthiosulfate reacts spontaneously with reduced glutathione at physiological pH, leading formation of S-allyl-mercapto-glutathione, radicals and peroxyl species, which able induce inhibition enzymes cysteine in catalytic site, such as sulfurtransferases. S-Allyl-mercapto-glutathione was purified and characterized by NMR MS, its cytotoxic effect 500 lM HuT 78 cells was analyzed, showing activation the p38–MAPK pathway. Many also to promote chemoprevention induction xenobiotic-metabolizing enzymes, inducing down-activation or detoxification carcinogens. Thus, S-allyl-mercapto-glutathione proteins involved in cellular system, S-transferase, have been evaluated both vitro cells. Although antitumor properties may arise from several mechanisms and it likely more events occur simultaneously, relevant role played glutathione conjugates radical species affect activity thiol-proteins involved fundamental processes.