Differences in the secretion pattern of oxidoreductases from Bjerkandera adusta induced by a phenolic olive mill extract.

摘要: Abstract The secretome of the white-rot fungus Bjerkandera adusta produced in synthetic Kirk medium was compared to that supplemented with an aqueous phenol-rich extract dry olive mill residues (ADOR). Distinct changes protein composition oxidoreductases, namely diverse class-II peroxidases and aryl alcohol oxidases were found. In ADOR-supplemented (ASC), 157 distinct proteins identified by analysis, whereas only 59 them without ADOR supplementation (Kirk culture; KM). Proteome analysis indicated number ASC more than doubled (from 4 11) KM. Two short manganese (MnP1 MnP6) one versatile peroxidase (VP1) represented 29% relative abundance (NSAF) ASC. VP1) also detected KM at a 3%. Further present lignin (LiP2), generic (GP) three dye-decolorizing (DyPs). DyPs (AAO) lower comparison addition peptide sequence secretion Mn2+-oxidizing as well AAOs followed enzyme measurement. activity increased nearly 30-fold 10 281 U l−1) after addition. enzymes responsible for successfully purified (BadVPI BadVPII). To prove potential involvement these degradation aromatic compounds, BadVPI tested its ability degrade recalcitrant dehydrogenated polymer (DHP, lignin). These results show natural materials act secretome-stimulating additives. Applying substances enables us investigate fungal detoxification processes gives insight into complexity secretomes, e.g. fungi.