Predominant role of hypoxia-inducible transcription factor (Hif)-1alpha versus Hif-2alpha in regulation of the transcriptional response to hypoxia.
作者： Heidi M Sowter , Peter J Ratcliffe , John W Moore , Raju R Raval , Adrian L Harris
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摘要: Tumor hypoxia induces the up-regulation of a gene program associated with angiogenesis, glycolysis, adaptation to pH, and apoptosis via hypoxia-inducible transcription factors (Hifs) 1 2. Disruption this pathway has been proposed as cancer therapy. Here, we use short interfering RNAs compare specific inactivation Hif-1alpha or Hif-2alpha show markedly different cell type-specific effects on expression migration. Remarkably, among panel genes, responses were critically dependent Hif-1 alpha but not Hif-2 in both endothelial breast cells renal carcinoma cells.
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