Amplification of DNA sequences in human multidrug-resistant KB carcinoma cells.

摘要: Abstract Four KB carcinoma cell lines selected independently for resistance to either colchicine, adriamycin, or vinblastine were studied. All showed high levels of the selecting drug and cross-resistance other drugs actinomycin D. Double-minute chromosomes could be identified on chromosomal spreads these multidrug-resistant lines. Amplification specific DNA sequences was demonstrated by using technique in-gel renaturation. share common amplified sequences. There are also that each line. A revertant line has reacquired sensitivity lost its Specific probes obtained cloning from in recognize all resistant The presence is strong evidence importance regions multiple resistance.