Ultrastructural analysis of interleukin-1 beta-induced leukocyte recruitment to the rat retina.

摘要: Purpose. To examine, by ultrastructural analysis th,e effect of th proinflammatore y cytokineinterleukin-1/? (IL-1/3 on) the integrity rat retina and blood-retinal barrier (BRB an)dto investigat e site leakage path leukocyte infiltration int thoe retina.Methods. Afte ar single injectio on f IL-1/3 into vitreous Lewi rats , recruit-ment retinal disease was assessed immediatel ay ant frequend t periods up to 14 days afterinjection light electron microscop andy immunohistochemistry . The ofthe BRB disruption were evaluated usine largge molecular weighttracer horseradish peroxidase phenotyp. recruited thse wa ass -sessed morpholog immunohistochemistry.Results. At 4 hours afte thr intravitrea l administratio IL-1/3n leukocyte, s observedinfiltrating retina. Leukocyte increased gradually peaked between 24 and48 after injection. Associated with this infiltrate wer ande fibrin edem leakagea indica-tive a breakdow inn BRBe Thi confirmed demonstratioe horseradishperoxidase extravasation acros retinas vascula bedr wit h leakag oef tracer throughdisrupted endothelial tight junctions. Using immunohistochemica anl d morphologic criteria,the majorit oyf infiltrating cells identifie ads monocytes-macrophages neutrophils;occasionally, T found. Ultrastructural analysi majorits showe oy celld thas tentered migrating through an thast there smallercontribution from ciliary body.Conclusions. vitreoue os Lewie ras cause as n acute,reversible inflammatory respons is accompaniee thatd breakdown vasculae rBRB. Interleukin-1/3 induces recruitmen ot mononuclear polymorphonuclear leuko-cytes that ente predominantly throug theh vasculatur aned appear tomigrate endothelia cellsl Thes. results suggest may be importantfactor in pathogenesie humas inflammation. Vi Invess Sci. Ophthalmol1996;38:25-35.JL he microenvironment parenchyma isregulated strictly variety protective mechanismsand structures. Principal among these presenceof cellular controls passage mole-cules hematogenous blood andthe neuroretina. This composed two celltypes, each at different anatomic location: vascu-lar vasculature