New equations describing the combined effect of pH and organic modifier concentration on the retention in reversed-phase liquid chromatography.

摘要: Six equations that express the combined effect of mobile phase pH and organic modifier content on sample retention in reversed-phase liquid chromatography (RPLC) are developed based either adsorption or partition model for retention. The tested against five data sets taken from literature. In tests two scales used, ws pH. It is shown a new seven-parameter equation works more satisfactorily, because it exhibits good numerical behavior, gives low values sum squares residuals represents experimental surfaces successfully. addition, danger overfitting, which leads to prediction physically meaningless surfaces, minimized by using proposed equation. Finally, possibility obtaining reliable pK weak acids bases chromatographically means derived also considered discussed.  2002 Elsevier Science B.V. All rights reserved.