Proximity to PML Nuclear Bodies Regulates HIV-1 Latency in CD4+ T Cells

摘要: Nuclear bodies (NBs), characterized by the presence of promyelocytic leukemia (PML) protein, are important components nuclear architecture, contributing to genetic and epigenetic control gene expression. In investigating mechanisms mediating HIV-1 latency, we determined that silenced but transcriptionally competent proviruses reside in close proximity PML NBs this association inhibits binds latent promoter, which coincides with inactive facultative heterochromatic marks, notably H3K9me2, at viral genome. degradation NB disruption result strong activation transcription as well release G9a, major methyltransferase responsible for loss heterochromatin marks from proviral DNA. Additionally, transcriptional requires displacement active actin polymerization. Thus, topology movement mediate regulation have implications controlling latency eradication.