Water mass-specificity of bacterial communities in the North Atlantic revealed by massively parallel sequencing.

摘要: Bacterial assemblages from subsurface (100 m depth), meso- (200-1000 depth) and bathy-pelagic (below 1000 zones at 10 stations along a North Atlantic Ocean transect 60°N to 5°S were characterized using massively parallel pyrotag sequencing of the V6 region 16S rRNA gene (V6 pyrotags). In dataset more than 830,000 pyrotags, we identified 10,780 OTUs which 52% singletons. The singletons accounted for less 2% OTU abundance, whereas 100 most abundant represented 80% 96% respectively all recovered OTUs. Non-metric Multi-Dimensional Scaling Canonical Correspondence Analysis excluding revealed clear clustering bacterial communities according water masses. More corresponded Proteobacteria 55% Alphaproteobacteria, mostly composed SAR11 cluster. Gammaproteobacteria increased with depth included relatively large number belonging Alteromonadales Oceanospirillales. bathypelagic zone showed higher taxonomic evenness overlying waters, albeit diversity was remarkably variable. Both low-abundance responsible distinct characterizing major deep-water Taken together, our results reveal that masses act as bio-oceanographic islands bacterioplankton leading mass-specific in deep waters Atlantic.