Coexpression of mRNAs for NGF, BDNF, and NT-3 in the cardiovascular system of the pre- and postnatal rat

摘要: The expression of NGF, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) mRNAs was examined in whole rat embryos the heart great vessels postnatal adult rats, using situ hybridization cRNA probes. patterns were correlated with innervation as revealed by immunostaining for neural cell adhesion molecule (NCAM) HNK-1 antibody, which demonstrates derivatives crest. neurotrophin mRNA localization different from those low-molecular-weight NGF receptor. Hybridization indicating presence all three neurotrophins is particularly prominent within tunica media aorta, pulmonary, other major elastic arteries thorax abdomen first observed on embryonic day 13 (E13) when being established rises to maximum E15. In fetus, there little or no detectable CNS PNS. NT-3 relatively constant high stages, while levels BDNF increase decrease over same time course. During fetal period, absent. additional label becomes coronary but not walls atria ventricles, than at base aorta pulmonary trunk.(ABSTRACT TRUNCATED AT 250 WORDS)