In vivo analysis of the 3' untranslated region of the hepatitis C virus after in vitro mutagenesis of an infectious cDNA clone.
作者： M. Yanagi , M. St. Claire , S. U. Emerson , R. H. Purcell , J. Bukh
关键词:
摘要: Large sections of the 3′ untranslated region (UTR) hepatitis C virus (HCV) were deleted from an infectious cDNA clone, and RNA transcripts seven deletion mutants tested sequentially for infectivity in a chimpanzee. Mutants lacking all or part terminal conserved poly(U–UC) unable to infect chimpanzee, indicating that both regions are critical vivo. However, third region, variable was able tolerate destroyed two putative stem–loop structures within this region. Mutant VR-24 containing proximal 24 nt UTR viable chimpanzee seemed replicate as well undeleted parent virus. The became viremic 1 week after inoculation with mutant VR-24, HCV genome titer increased over time during early acute infection. Therefore, but not seem be vivo HCV.
harvard.edu
core.ac.uk
sci-hub.se 参考文章(33)
S. Dash, M. A. Gerber, N. Hiramatsu, H. Tsuji, A. B. Halim, Transfection of HepG2 cells with infectious hepatitis C virus genome. American Journal of Pathology. ,vol. 151, pp. 363- 373 ,(1997)
S. E. Behrens, L. Tomei, R. De Francesco, Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus. The EMBO Journal. ,vol. 15, pp. 12- 22 ,(1996) , 10.1002/J.1460-2075.1996.TB00329.X
Christian W. Mandl, Heidemarie Holzmann, Tamara Meixner, Susanne Rauscher, Peter F. Stadler, Steven L. Allison, Franz X. Heinz, Spontaneous and Engineered Deletions in the 3′ Noncoding Region of Tick-Borne Encephalitis Virus: Construction of Highly Attenuated Mutants of a Flavivirus Journal of Virology. ,vol. 72, pp. 2132- 2140 ,(1998) , 10.1128/JVI.72.3.2132-2140.1998
R Men, M Bray, D Clark, R M Chanock, C J Lai, Dengue type 4 virus mutants containing deletions in the 3' noncoding region of the RNA genome: analysis of growth restriction in cell culture and altered viremia pattern and immunogenicity in rhesus monkeys Journal of Virology. ,vol. 70, pp. 3930- 3937 ,(1996) , 10.1128/JVI.70.6.3930-3937.1996
A A Kolykhalov, S M Feinstone, C M Rice, Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA. Journal of Virology. ,vol. 70, pp. 3363- 3371 ,(1996) , 10.1128/JVI.70.6.3363-3371.1996
Noriko Yamada, Kiyomi Tanihara, Akira Takada, Tetsuya Yorihuzi, Mikihiro Tsutsumi, Hiroyuki Shimomura, Takao Tsuji, Takayasu Date, Genetic Organization and Diversity of the 3′ Noncoding Region of the Hepatitis C Virus Genome☆ Virology. ,vol. 223, pp. 255- 261 ,(1996) , 10.1006/VIRO.1996.0476
M. Yanagi, R. H. Purcell, S. U. Emerson, J. Bukh, Transcripts from a single full-length cDNA clone of hepatitis C virus are infectious when directly transfected into the liver of a chimpanzee Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 8738- 8743 ,(1997) , 10.1073/PNAS.94.16.8738
J H Hoofnagle, Hepatitis C: the clinical spectrum of disease. Hepatology. ,vol. 26, pp. 15- 20 ,(1997) , 10.1002/HEP.510260703
Christian W. Mandl, Judith H. Aberle, Stephan W. Aberle, Heidemarie Holzmann, Steven L. Allison, Franz X. Heinz, In vitro -synthesized infectious RNA as an attenuated live vaccine in a flavivirus model Nature Medicine. ,vol. 4, pp. 1438- 1440 ,(1998) , 10.1038/4031
J. Bukh, R. H. Purcell, R. H. Miller, Sequence analysis of the 5' noncoding region of hepatitis C virus. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 4942- 4946 ,(1992) , 10.1073/PNAS.89.11.4942