Collagenase in Human Cornea: Immunologic Localization

作者: James M. Gordon

DOI: 10.1001/ARCHOPHT.1980.01020030337022

关键词:

摘要: • Collagenase has been localized in human corneas using immunocytochemical techniques employing a specific antiserum to skin collagenase. The enzyme was found vivo ulcerating corneas, whereas obtained from variety of nonulcerative conditions, no immunologic evidence the seen. Corneal collagenase present only stromal portion cornea, suggesting that cells this tissue are source enzyme. localization corneal stroma reproduced by placing either or nonulcerating culture, indicating organ culture system may represent model duplicates some conditions disease.

参考文章(24)
Stuart I. Brown, Richard M. Hook, Carol Weller Hook, Fibroblast collagenase partial purification and characterization. Investigative Ophthalmology & Visual Science. ,vol. 12, pp. 771- 776 ,(1973)
Eugene A. Bauer, Arthur Z. Eisen, John J. Jeffrey, Radioimmunoassay of Human Collagenase Journal of Biological Chemistry. ,vol. 247, pp. 6679- 6685 ,(1972) , 10.1016/S0021-9258(19)44745-5
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
B. Johnson-Muller, J. Gross, Regulation of corneal collagenase production: epithelial-stromal cell interactions Proceedings of the National Academy of Sciences of the United States of America. ,vol. 75, pp. 4417- 4421 ,(1978) , 10.1073/PNAS.75.9.4417
George P. Stricklin, Eugene A. Bauer, John J. Jeffrey, Arthur Z. Eisen, Human skin collagenase: isolation of precursor and active forms from both fibroblast and organ cultures. Biochemistry. ,vol. 16, pp. 1607- 1615 ,(1977) , 10.1021/BI00627A013
Michael Berman, Claes H. Dohlman, Marcel Gnädinger, Peter Davison, Characterization of collagenolytic activity in the ulcerating cornea. Experimental Eye Research. ,vol. 11, pp. 255- 257 ,(1971) , 10.1016/S0014-4835(71)80030-1
H. H. Slansky, M. C. Gnadinger, M. Itoi, C. H. Dohlman, Collagenase in Corneal Ulcerations Archives of Ophthalmology. ,vol. 82, pp. 108- 111 ,(1969) , 10.1001/ARCHOPHT.1969.00990020110026
J. P. McCulley, H. H. Slansky, D. Pavan-Langston, C. H. Dohlman, Collagenolytic activity in experimental herpes simplex keratitis. Archives of Ophthalmology. ,vol. 84, pp. 516- 519 ,(1970) , 10.1001/ARCHOPHT.1970.00990040518023
Eugene A. Bauer, James M. Gordon, Max E. Reddick, Arthur Z. Eisen, Quantitation and immunocytochemical localization of human skin collagenase in basal cell carcinoma. Journal of Investigative Dermatology. ,vol. 69, pp. 363- 367 ,(1977) , 10.1111/1523-1747.EP12510240
Gerald S. Lazarus, John R. Daniels, Robert S. Brown, Howard A. Bladen, Harold M. Fullmer, Degradation of collagen by a human granulocyte collagenolytic system Journal of Clinical Investigation. ,vol. 47, pp. 2622- 2629 ,(1968) , 10.1172/JCI105945