The VP1 S154D mutation of type Asia1 foot-and-mouth disease virus enhances viral replication and pathogenicity.

摘要: One of the proteins encoded by foot-and-mouth disease virus (FMDV), VP1 protein, a capsid plays an important role in integrin receptor attachment and humoral immunity-mediated host responses. The recognition motif antigenic epitope exist within G-H loop, which is comprised amino acids 134-160 protein. FMDV strain, Asia1/HN/CHA/06, isolated from pig, was passaged four times suckling mice sequenced. Sequencing analyses showed that there mutation Arg-Gly-Asp/Arg-Asp-Asp (RGD/RDD, 143-145) 154 serine/Asp (VP1 S154D) loop influence RGD/RDD on Asia1 phenotype has been previously studied. In this study, to determine S154D replication pathogenicity, two recombinant FMDVs with different residues only at site were rescued reverse genetics techniques their infectious potential cells pathogenicity pigs compared. Our data indicates increases level Asia1/HN/CHA/06 BHK-21, IB-RS-2, PK-15 enhances pigs. Through transient transfection-infection assay compare usage viruses, result shows markedly ability type use receptors αυβ6 αυβ8 pig. This study identifies key research target for illuminating located pathogenicity.