Immune Cell Responses to the Cattle Lungworm, Dictyocaulus viviparus

摘要: The cattle lungworm, Dictyocaulus viviparus, is a parasitic nematode, which can cause severe pulmonary disease. Repeated natural infections result in protective immunity, and live attenuated vaccine exists. However, vaccines have several disadvantages, alternative control methods are needed. Awareness of increasing anthelmintic resistance makes parasite with reduced reliance on chemotherapeutics preferable, an effective modern to D. viviparus based recombinant antigens would provide valuable alternative. To enable the design such vaccine, identification immune mechanisms behind fundamental. overall aim this thesis was study cellular responses viviparus. In order identify cells involved development mononuclear cell populations lungs experimentally infected calves were studied during two subsequent infections. A large influx activated γδ T observed both after primary infection reinfection, suggests role for response vitro analyses lymphocyte revealed that from naive animals induced proliferate. Cells expressing CD4, CD8 as well TCR responded, suggesting worms contain substances broad mitogenic effect. When investigating collected animals, no clear acquired detected. Investigations into nature components indicated they low molecular mass immunogenic proteins so far unknown identities.