Transforming growth factor-beta 1 modulates the expression of class II histocompatibility antigens on human cells.

摘要: We have examined the effects of transforming growth factor-beta (TGF-beta 1) on expression class II histocompatibility Ag, HLA-DR: induced by human rIFN-gamma (rHuIFN-gamma) in melanoma, Hs294T cells and 2) constitutively expressed a subclone cell line. The HLA-DR Ag was rHuIFN-gamma dose- time-dependent manner with maximal levels obtained 10 ng/ml after 48 h exposure. Treatment natural porcine platelet-derived or rTGF-beta 1 (1 to 100 ng/ml) presence (0.1 reduced percentage positive for this approximately 40 50%, as determined flow cytometry. This suppressive effect TGF-beta not restricted inasmuch surface PBMC-derived adherent also significantly inhibited 1. Northern blot analysis cytoplasmic RNA extracted from indicated that decreased cell-surface correlated mRNA transcripts encoding Ag. effectively had been pretreated were expressing antigen without exogenous treatment.