Glucose-free fructose production from Jerusalem artichoke using a recombinant inulinase-secreting Saccharomyces cerevisiae strain

摘要: Purpose of work Using inulin (polyfructose) obtained from Jerusalen artichokes, we have produced fructose free residual glucose using a recombinant inulinase-secreting strain Saccharomyces cerevisiae in one-step fermentation Jerusalem artichoke tubers. For producing inulin, inulinase-producing Saccharomyce was constructed with deficiency uptake by disruption two hexokinase genes hxk1 and hxk2. The inulinase gene introduced into S. cloned Kluyveromyces cicerisporus. Extracellular activity the hxk-mutated reached 31 U ml−1 after 96 h growth. When grown medium containing tubers as sole component without any additives, yeast accumulated up to 9.2% (w/v) broth only 0.1% left 24 h.