Multiplex Amplification and Typing Procedure for the Loci D1S80 and Amelogenin

作者: Bruce Budowle , Barbara W. Koons , Jacqueline D. Errera

DOI: 10.1520/JFS13973J

关键词:

摘要: A method has been developed that enables multiplex amplification and simultaneous typing of the loci D1S80 amelogenin using discontinuous polyacrylamide gel electrophoresis silver staining. The protocol is sensitive, simple, rapid, relatively inexpensive. results analysis were comparable to those obtained when each locus was analyzed individually. small validation study undertaken evaluate forensic applicability this system. data demonstrate DNA exposed a variety environmental insults yields reliable results.

参考文章(27)
Lucia Casarino, Francesco De Stefano, Armando Mannucci, Marcello Canale, HLA-DQA1 and Amelogenin Coamplification: A Handy Tool for Identification Journal of Forensic Sciences. ,vol. 40, pp. 456- 458 ,(1995) , 10.1520/JFS13803J
Bruce Budowle, F. Samuel Baechtel, Jill B. Smerick, Kathryn W. Presley, Alan M. Giusti, Gregory Parsons, Martin C. Alevy, Ranajit Chakraborty, D1S80 population data in African Americans, Caucasians, southeastern Hispanics, southwestern Hispanics, and Orientals Journal of Forensic Sciences. ,vol. 40, pp. 38- 44 ,(1995) , 10.1520/JFS13757J
Bruce Budowle, R. M. Fourney, L. A. Presley, J. S. Waye, G. G. Shutler, A simple and sensitive method for quantifying human genomic DNA in forensic specimen extracts BioTechniques. ,vol. 7, pp. 852- 855 ,(1989)
Susan Scholl, Bruce Budowle, Kristin Radecki, Mark Salvo, NAVAJO, PUEBLO, AND SIOUX POPULATION DATA ON THE LOCI HLA-DQA1, LDLR, GYPA, HBGG, D7S8, GC, AND D1S80 Journal of Forensic Sciences. ,vol. 41, pp. 47- 51 ,(1996) , 10.1520/JFS13895J
Kwang Man Woo, Bruce Budowle, Korean population data on the PCR-based loci LDLR, GYPA, HBGG, D7S8, Gc, HLA-DQA1, and D1S80. Journal of Forensic Sciences. ,vol. 40, pp. 645- 648 ,(1995) , 10.1520/JFS13842J
Catherine Theisen Comey, Barbara W. Koons, Kathryn W. Presley, Jill B. Smerick, Carl A. Sobieralski, Donna M. Stanley, F. Samuel Baechtel, DNA Extraction Strategies for Amplified Fragment Length Polymorphism Analysis Journal of Forensic Sciences. ,vol. 39, pp. 13711J- 1269 ,(1994) , 10.1520/JFS13711J
Manfred N. Hochmeister, Bruce Budowle, Urs V. Borer, Urs Eggmann, Catherine T. Comey, Richard Dirnhofer, Typing of deoxyribonucleic acid (DNA) extracted from compact bone from human remains. Journal of Forensic Sciences. ,vol. 36, pp. 1649- 1661 ,(1991) , 10.1520/JFS13189J
F. Samuel Baechtel, Kathryn W. Presley, Jill B. Smerick, D1S80 typing of DNA from simulated forensic specimens. Journal of Forensic Sciences. ,vol. 40, pp. 536- 545 ,(1995) , 10.1520/JFS13822J
Susan Cosso, Rebecca Reynolds, Validation of the AmpliFLP D1S80 PCR Amplification Kit for forensic casework analysis according to TWGDAM guidelines. Journal of Forensic Sciences. ,vol. 40, pp. 424- 434 ,(1995) , 10.1520/JFS13797J