Toxoplasma gondii infection of fibroblasts causes the production of exosome-like vesicles containing a unique array of mRNA and miRNA transcripts compared to serum starvation

摘要: Background : Until recently thought to be of little significance unless occurring during pregnancy, Toxoplasma gondii infection human hosts is now known play a larger role in mental health and growing concern the care community. We sought elucidate possible mechanism by which may cause some behavioural pathology associated with infection. hypothesized that exosomes playing role. Methods utilized electron microscopy detect presence size extracellular vesicles supernatants Toxoplasma-infected foreskin fibroblasts (HFF). then microarray analysis discern mRNA miRNA content isolated from (Toxo) serum-starved (SS) HFF. Results recovered consistent we called exosome-like (ELVs) SS Toxo cultures. The these ELVs was highly regulated creating specific unique expression profiles comparing ELVs, RNA whole cell homogenates. Interestingly, among most enriched cells are 4 species have been described literature as having neurologic activity: Rab-13, eukaryotic translation elongation factor 1 alpha 1, thymosin beta LLP homolog. In addition, uniquely expressed include miR-23b, well-known regulator IL-17. Conclusion While production containing mRNAs modify behaviour reported pathology, enrichment ultimate vivo effect remains investigated. Keywords: Toxoplasma; mRNA; miRNA; exosomes; vesicles; (Published: 11 December 2013) Citation: Journal Extracellular Vesicles 2013, 2 22484 - http://dx.doi.org/10.3402/jev.v2i0.22484 To access supplementary material this article, please see Supplementary files under Article Tools online.