Experimental Estimation of Mutation Rates in a Wheat Population With a Gene Genealogy Approach

摘要: Microsatellite markers are extensively used to evaluate genetic diversity in natural or experimental evolving populations. Their high degree of polymorphism reflects their mutation rates. Estimates the rates therefore necessary when characterizing As a complement classical designs, we propose use populations, where initial state is entirely known and some intermediate states have been thoroughly surveyed, thus providing short timescale estimation together with large number cumulated meioses. In this article, derived four original gene genealogy-based methods assess limited bias due relevant model assumptions incorporating state, new alleles, effective population size. We studied evolution at 21 microsatellite markers, after 15 generations an wheat population. Compared parents, 23 alleles were found generation 9 loci studied. provide evidence that they arose by mutation. Corresponding estimates ranged from 0 4.97 × 10−3 per (i.e., year). Sequences several revealed length was only variation core microsatellite. Among different characteristics, both motif repeat independent Nei correlated novel diversity. Despite reduced size, global increased population, suggesting should be caution as indicator biodiversity conservation issues.