Expression of T-cell differentiation antigens on effector cells in cell-mediated cytotoxicity in vitro. Evidence for functional heterogeneity related to the surface phenotype of T cells.

摘要: The cell-mediated cytotoxicity (CMC) of nonadherent cells from the peritoneal cavity (NAPC) alloimmunized mice can be measured by [3H]proline microassay. exhibition thymus-derived (T) cell antigens on these killer was studied incubating them with relevant T-cell antisera and complement (C), under optimal conditions for lysis, before performance CMC assay. Under conditions, following were demonstrable population in terms percent reduction respective antisera: (a) Thy-1.1 (83%) Thy-1.2 (100%), (b) MSLA (86%), (c) NTA-RA (a antigen recognized naturally occurring autoantibody NZB mice) (62%), (d) Ly1.1 )58%, (e) Ly-2.1 (11%; considered a marginal result) Ly-2.2 (63%), (f) Ly-3.2 (77%). not demonstrable: (g) TL, (h) Ly-1.2. (i) Ly-3.1 studied. Omission C deprived all tested their capacity to suppress CMC, indicating that components such may perform no direct function CMC. On assumption Ly+ are Thy-1+, it is clear members immune NAPC must heterogenous. This follows fact proportions T lysed different Ly did correspond ensuing degree loss capacity. extremes represented anti-Ly-1.2 (74% Thy-1+ lysed, but CMC) (54% 77% CMC). From this initial survey appears C57BL/6 active vitro relatively rich surface Ly-2/Ly-3 category poor representation Ly-1 antigens. It remains seen whether phenotype, Ly-2/Ly-3, characteristic mouse generally. results subsets immunological functions exhibit qualitative or quantitative differences specified loci; will easier assess future when experiments same dealing other than become available.