Overexpression of mitogen-activated protein kinase (ERK1) enhances T- cell cytokine gene expression: role of AP1, NF-AT, and NF-KB

摘要: Transfected Jurkat cells overexpressing extracellular signal-regulated kinase (ERK1), also referred to as mitogen-activated protein (MAP) kinase, were selected by Western blotting assay using anti-ERK1 and antiphosphotyrosine antibodies in combination with a functional MAP assay. We then asked whether enhanced ERK1 expression had any effect on induction of T-cell cytokine genes. The results show that overexpression enhances interleukin-2 (IL- 2), IL-3, granulocyte-macrophage colony-stimulating factor mRNA; no change was seen the alpha-actin gene. DNA-binding activities transcription factors AP1, NF-AT, NF-kB specifically increased twofold fourfold ERK1-overexpressing clones relative nontransformed or vector-transformed cells, whereas enhancement CK1-CK2 DNA binding activity detected after overexpression. Additionally, NF-AT associated transactivating cells. These provide direct evidence for role regulation gene indicate such is likely mediated through specific nuclear factors.