Structural and Functional Association of Trypanosoma brucei MIX Protein with Cytochrome c Oxidase Complex
作者： Alena Zíková , Aswini K. Panigrahi , Alessandro D. Uboldi , Rachel A. Dalley , Emanuela Handman
DOI: 10.1128/EC.00204-08
关键词:
摘要: A mitochondrial inner membrane protein, designated MIX, seems to be essential for cell viability. The deletion of both alleles was not possible, and the a single allele led loss virulence aberrant segregation division in Leishmania major. However, mechanism by which MIX exerts its effect has been determined. We show here that is also expressed mitochondrion Trypanosoma brucei, using RNA interference, we found leads phenotype similar described Leishmania. had major on cytochrome c oxidase activity, potential, production ATP oxidative phosphorylation. Using tandem affinity purification tag, associated with multiprotein complex contains subunits (respiratory IV), composition characterized detail. specific function unknown, but it appears important IV T. brucei.
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